Breast cancer in women pose a problem, and it can be difficult at times to evaluate the possibility of the cancer recurrence in early cancer. The probability of the cancer recurrence in early breast cancer is not rare, so we studied the major
role
of
the gene mutation-especially p53-as a important prognostic factor to predict the cancer recurrence.
There is increasing evidence relating todevelopment andprogression of cancer to an accumulation of mutation at the genomic level. The most frequently mutated gene known to data in sporadic breast cancer appears to be the tumor suppressor gene
p53.
A
mutation affecting the codon sequence of the p53 gene consequently leads to the loss of its negative growth control properties. Presently available results have been indicated that mutations teend to cluster in a protion of gene spanning codon
132
to
281(corresponding to exon 5, 6, 7, 8, and 9) known to include four highly conserved block and these exons have been shown to comprise highly conserved sequences and believed to be the target for over 90% of the acquired mutations in human cancer,
and
the majority localized in exon 5, 7, and 8. The author examined the p53 gene in genomic DNA samples from 46 primary breast cancer tissues fixed in formalin and embedded in paraffin which were followed up more than 36 months. Using PCR-SSCP
approach, the
author analyzed exon 5 of p53 for detecting mutations by non-isotopic ethidine bromide staining, then analyzed the mutations by sequencing using Sequenase PCR Product Sequencing Kit (USB, Product No. 70170, USA). The exon 5 of p53 gene was
amplified by
PCR as 211bp. Five of 46(10.8%) mutations in exon 5 of p53 gene were detected by non-isotopic SSCP approach in PCR products. The mutations were analyzed by sequencing using PCRproduct and four of all were succeeded. All of them were frameshift
mutations, and all mutations were deletion of C signle base in codon 156, and stop codon in codon 169. The toehr available western results have been the missense mutations in G, C paris.
In conclusion, the sequencing analysis of mutant PCR product propose carefully the probability that the mutant biology of breast cnacer was different in Korean women in comparison to Western women. It is necessary to perform further sutdy in
mutation of
other exons, 2, 6, 7, 8, 9, and 11 of p53 gene.
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